Análisis funcionales y genéticos en microorganismos de interés del cepario de Corpogen

Abstract

Antimicrobial resistance is a global public health problem, which has motivated the search for new molecules that contribute to the treatment of infections caused by microorganisms. Actinobacteria from undisturbed tropical environments and in association with lichens, thanks to their microbial adaptations such as the presence of resistance genes and bioactive molecules, become an alternative for the search for antimicrobial compounds. For this reason, in nine bacteria from the CorpoGen strain collection, their growth in culture media supplemented with antibiotics and the presence of the VanA and VanB genes were evaluated by PCR technique, and their functional potential was also characterized in order to contribute to the studies with multi-drug resistant microorganisms. Functional analyzes with the supernatants of Staphylococcus sp. (19) and Bacillus sp. (55) inhibited the growth of E. coli by 92 and 95% at 60 hours of incubation. Solid resistance studies also found that the actin Streptomyces avidnii (CG885) grew in Meropenem and that many actins showed inhibition. for other bacteria studied here with the double layer method, where Arthrobacter sp. (PNE1-1) presents an important functional potential. Both the in vitro antimicrobial activity of these isolates and the presence of antibiotic resistance genes indicate genomic diversity and functional capacity associated with the production of bioactive metabolites in some bacteria of the strain. This work confirms the importance of studying environmental microorganisms, their functional potential and searching for new antimicrobial compounds produced by them.