Estudio y análisis funcional: Identificación de ARNc Y ARNlnc en tejidos meristemáticos dentro del transcriptoma de clones de Solanum tuberosum del grupo Phureja colombiana

Abstract

Solanum tuberosum Group Phureja, commonly known as papa criolla, is a key crop in Colombia due to its distinctive traits. In this study, solid mutants generated by Cobalt-60 mutagenesis, specifically MV6-17, MV6-39, and MV6-43, were evaluated alongside their progenitor (“Flor Blanca”) and the control (“Cultivar Criolla Colombia”). RNA-Seq characterization enabled the transcriptomic profiling of each individual, identifying both coding RNAs and long non-coding RNAs (lncRNAs). The analysis revealed significant interactions between coding RNAs and lncRNAs associated with molecular and cellular functions, including transcripts related to phytohormone-mediated regulation of dormancy. The bioinformatic workflow included quality control using FastQC v0.12.1 (Andrews, 2010), read trimming with Trimmomatic v0.39 (Bolger et al., 2014), alignment to the DM 1-3 516 R44 v.6 reference genome (from SpudDB) via TopHat2 v2.1.1 (Trapnell et al., 2009), transcript assembly with Cufflinks v2.1.1 (Trapnell et al., 2012), lncRNA identification using AWK v5.2.1 (Aho et al., 1988) and CPC v2.0 (Kang et al., 2017), co-expression network construction using PyWGCNA v2.0.0 (Rezai et al., 2023), and functional enrichment analysis with G:Profiler. This research, conducted within the BIOMOLc group at Universidad Distrital, focused on identifying co-expression relationships in papa criolla mutant clones. Findings were disseminated at national and international conferences, contributing to the advancement of bioinformatic applications in this crop.