Análisis de transcriptoma y anotación funcional de genes relacionados con dormancia en papa criolla Solanum tuberosum vf.phureja irradiada con cobalto 60

Abstract

The creole potato generally presents a dormancy period, among its advantages we find that it is a process of vital importance for survival in an unfavorable period and without optimal conditions, but in the post-harvest period, it becomes a negative factor that affects the viability of the product at the commercial level. In potato, dormancy gives rise to other processes such as tuberization, which is defined as a period where no visible sprout growth process takes place, and implies a correlated biological relationship in the tuber. This study focused on analyzing and characterizing the transcriptome of the creole potato, and relating it to the genes that affect dormancy processes in creole potato solanum tuberosum vf. Phureja and that have been the subject of study in the Molecular Biology seedbed, a bioinformatics protocol was implemented, which consisted of a referenceguided assembly of the transcriptome with the Cufflinks repertoire, differential expression analysis, mapping with the genome reported in Spud and finally, annotation and quality assessment of the assembly was performed with Busco, to review databases such as TAIR and Swissprot (homology-based annotation). All the bioinformatic analysis of the RNA-seq libraries was developed in the server of the Office of Research Computing, generating a descriptive protocol for in bash language and executable in a Slurm environment, and all the processing involved in working with these data.