Estudio del metabolismo del NAD+en protozoos de vida libre y parásitos

Abstract

The study of the metabolism of NAD+ in protozoos of free-living and parasites, constitutes a metabolic approach of the NMNAT in function with the type of life of these organisms. This is of great interest, taking into account that many of these protozoa generate high impact global diseases and treatments are not efficient, whereupon it is necessary to propose new drug targets for the development of strategies more effective. In this work through a bioinformatic approach were identified new 18 of NMNAT of protozoos, which presented sequences amino acid highly conserved along with its binding domains to ATP. Were selected the candidates NMNAT of the parasite extracellular Trichomonas vaginalis, because that is the cause of higher disease of transmission sexual (ETS) no viral with 276 million global cases a year and the generation of resistant strains against the drugs used, for these candidates was carried out further study in silico and experimental approach, where they were cloned and expressed the NMNAT of T. vaginalis, thus constituting the first step to study the metabolism of NAD+ in this parasite.