Estudio fotoquímico de hojas de la especie Cavendishia compacta (Ericaceae) y evaluación de su actividad antiinflamatoria

Abstract

This paper describes the results of the phytochemical study of the ethanolic extract of leaves of the specie classified as Cavendishia compacta under number 574699 COL at the National Colombian Herbarium, in order to contribute to research phytochemical in the Ericaceae family and gender, because this gender does not report phytochemical studies. The methods used to get the extracts and fractions were: Column Chromatography (CC), Thin Layer Chromatography (TLC) and Preparative Thin Layer (PTLC), yielded a mixture type diterpene of compounds formed by rimueno, biformeno and kaurano (9,2 mg), a mixture of aromatic compounds consisting of benzaldehyde and acetophenone (21,6 mg), a mixture of compounds consisting of type triterpene α-amyrin and β-amyrin (30 mg) and the isolation of two flavonoids: morin (87 mg) and myricetin (160 mg). The structural elucidation of mixtures and isolated compounds were carried out using techniques GC-MS and 1H NMR. From fresh leaves of the specie Cavendishia compacta was obtained essential oil with a yield of 0.27% (m/v) by stripping with water vapor. Its characterization was performed (GC-MS), identification is performed by comparing retention indices (IR) and Mass Spectra Library (NIST 08). 22 components were identified which constitute 77% of the total oil composition on being the sesquiterpene caryophyllene the highest percentage. The anti-inflammatory activity was evaluated for E.EtOH.H, F.HEX.CcH, F.CH2Cl2.CcH and F.AcOEt.CcH, using the model of ear edema induced by 12- 13 Tetradecanoylphorbol acetate (TPA), being the E.EtOH.H and F.CH2Cl2.CcH containing the mixture of terpenes and flavonoids, which showed moderate antiinflammatory effect of 49.3% and 39.8% respectively.