Perfilado metabólico de ejemplares de género Lupinus (Fabaceae) presentes en Cundinamarca y Boyacá

Abstract

Colombia has an inestimable wealth plant and have overlooked the valuable contributions that plants can provide the economic, social and research development. Therefore, the study of the genus Lupinus was made, because it is distributed in the country widely plants, but gender does not have chemotaxonomic information and phylogenetics, the generation of this document still needed, shows the metabolomic profiling made to copies of genus Lupinus, plants belonging to the family Fabaceae, starting from the ethanol extracts of leaves, stems, flowers, pods and seeds; for this, initially the chemical profile was determined by quantification of phenols (CFT), flavonoids (FL) and free radical scavenging capacity (CCRL). Also, the extracts by HPLC were analyzed in order to identify the detectable secondary metabolites in each plant organ. Quantitative analysis a significant correlation between the CFT and FL was not evidenced with reagent DPPH in almost all organs of study, except that which showed the seeds between the CFT and CCRL (ccP=0.60). With HPLC-MS were achieved to detect 28 compounds in total, 23 in leaves, 20 in stems, 14 in flowers, 17 in pods and 21 in seeds; the tentative identification allowed to find 27 flavonoids and an alkaloid. Finally was PCA with HCA validating differences and similarities along with discrimination through Orthogonal Partial Least Squares, supervised by the CFT, FL and CCRL. In conclusion in genus Lupinus can be cataloged as versatile , since 5 species showed changes in the chemical composition and content of secondary metabolites depending on the place, conditions of each plant, their environment and time of year, whether producing or not certain compounds that help you survive.