Impacto en la Producción de Lípidos por Scenedesmus dimorphus en Medio de Cultivo BG-11 Suplementado con Vitamina B3 o Vitamina B9

Abstract

The genus Scenedesmus can be found in bodies of fresh water around the world, and even on the ground. Its cosmopolitan nature illustrates the wide range of environmental conditions that these organisms can tolerate, making use of their phenotypic plasticity. Due to its simple nutritional requirements and rapid growth rates, the Scenedesmus culture is easy to maintain in the laboratory (Vazquez, 2011). In controlled crop media, its growth depends on several factors such as; water, temperature, CO2, light, mineral salts among others, for which a vertical semi-continuous bioreactor was designed with a lux intensity of 2,800 and a temperature in the range of 18 ° C to 28 ° C with a photoperiod of 18 light hours - 6 hours night, which allowed a comparison of the lipids obtained from the microalga Scenedesmus dimorphus of the BG-11 culture medium and two supplemented, one with vitamin B3 (VB3) and the other with vitamin B9 (VB9). For the extraction the Bligh and Dyer method was used, modified by Arias et al (2011) and modified by García, K and Novoa, J (2019) for the analysis of the extraction gas chromatography was used coupled to mass spectrometry throwing lipids present in the three culture media (Control, VB9 and VB3), these presented in common terpenes: (Neophytadiene and Phytol), while lipids such as Stigmast - 7- en-3-ol (3. Beta., 5 alpha, 24S and Chondrillasterol are only present in the control media and VB3, finally the fatty acid 9, 12, 15 - Octadecatrienoic acid, (Z, Z, Z) - and Ergost - 7 - in - 3 - ol alone in the medium VB3