Síntesis de péptidos con y sin restricción conformacional de las proteínas HPV16 L1 y HPV16 E7, para su posterior estudio de reactividad

Abstract

At present, we have generated about 500,000 annual deaths worldwide due to cervical cancer, problems of health that also reached the social sphere, together with its predecessor, the human papilloma virus, so it is necessary to start to generate new methods of treatment and diagnosis of these. The various studies on peptides have led to believe that the design of key sequences of native proteins, can be the solution to generate structural pampering that generate a large immune response against viruses that generate serious illnesses. According to the above this research was conducted, starting with finding the L1 and E7 of human papillomavirus genotype 16 virus proteins in the database Protein Data Bank (PDB), these data were worked on I-TASSER, where one of these blast was performed and an approximation of the tertiary structure generated, this was analyzed and fragments were determined with conformational restrictions are exposed and exhibit high antigenicity. HPV16 E7 protein helices 1 and 3 present were chosen between residues 7-16 and 74-84 respectively. HPV16 L1 protein helices 2 and 4 present were chosen between residues 385-394 and 414-425 respectively and FG and HI loops being residues 273-284 and 348-360 respectively. thus obtaining a total of 6 peptides which were synthesized with their respective linear conformational constraint and its counterpart by peptide synthesis strategy Fmoc solid phase. To generate the proper conformational constraints of each of the sequences the nucleation site JAZ was used (patent pending by the University District and Poteoma group), which managed to stabilize alpha-helix conformations and loop of different sequences short. Of each of the peptides synthesized analysis was performed by HPLC, mass circular dichroism, whereby obtaining the desired peptides as evidenced the formation and stabilization of the corresponding conformational constraints, most purity levels and percentages high yields.