Evaluación del Rol de la Proteína de Andamio Kidins220/Arms en el Efecto Estimulante del BDNF Sobre la Expresión y Estabilización de Proteínas de la Sinapsis GABAérgica

Abstract

The GABAergic synapses involved in inhibition of the central nervous system, is compromised in some pathologies as epilepsy and schizophrenia. The not production of GABA neurotransmitter for GAD65 enzyme or defects in the anchoring of the GABAA receptors due to Gephyrin scaffolding protein, are among the causes. It has been demonstrated that BDNF has a preponderant role in the regulation of the expresion of these proteins through the pathway of MAPK. However this pathway be activated for Sch or ARMS proteins. To determine whether ARMS Participates in the BDNF’s signaling pathway or not, we made hipocampal neurons cultures of rat embryos, transfected with shARMS plasmid, stimulated with BDNF and fixed to Immunocytochemistry of GAD65 and Gephyrin. Besides we evaluated the overexpression of ARMS in the regulation of the clustering of Gephyrin in HEK cells. We found that BDNF induced an increase in the expression of GAD65 and Gephyrin, as well as in the GABAergic synapses density, nevertheless when the ARMS levels decrease this leads to changes in the basal expression of GAD65 and in the stimulant effect of BDNF, Our results suggests that ARMS might be involved in the regulation of GAD65 expression and in the clustering of Gephyrin mediated for BDNF