Medición de la degradación de polietilenglicol 400 por consorcio Pseudomonas sp. y Rhizobium trifolii empleando demanda química de oxígeno

Abstract

Bacteria of the genus Pseudomonas sp. and Rhizobium trifolii, which have a high degradation capacity of polymer-derived contaminants, were inoculated in a medium of minimum salts with PEG 400 (molecular weight polyethylene glycol 400) as a limiting substrate and subjected to peptone variables, pH, PEG 400, stirring and temperature to find the optimal growth conditions. Three Batch reactors were mounted; BK1 control system with 0,1% PEG 400 and 0,1% peptone as growth driver, BK2 with 0,1% PEG 400, BK3 with culture medium without inoculum (used as blank) and, were taken fourteen samples in 20 days to determine the degradation of PEG 400 by means of the chemical oxygen demand (COD) method in a closed system. In BK1 a 98,5% decrease in polymer was obtained, which showed that this symbiosis was effective in achieving the removal of the contaminant.