Uso del estimador de Kaplan - Meier para la comparación de envejecimiento celular bajo diferentes condiciones en sistemas biológicos

Abstract

Experimentation from individual cells allows us to obtain information on cellular aging. The. cerevisiae ”in particular is one of the most basic models for this type of study and research; Recent works show devices and methods that bring with them limitations in the manufacture of the required elements, in addition to subjecting the cells to mechanical pressures without knowing whether or not an internal affectation occurs in them, that is why the construction of a hydrodynamic entrapment device, low cost, easy to build and with the advantage of reducing said pressure, thus involving physical models and principles, such as The Kaplan Meier Estimator in statistical physics, all this for single-cell studies of yeast populations during Long periods of time.

The project is developed in different stages, the first consists of the yeast cell growth that takes approximately two days, the second stage accounts for the manufacture of the chip, cleaning and assembly process, the third stage consists of the functional tests of the device and its accessories in general, in the fourth stage the device is installed in the microscope, photographic capture, operating time and data collection after its completion, finally in the last stage the collected images are reproduced , data selection, graphing and analysis.

For the development of the last stage, statistical physics is used, especially the Kaplan Meier estimator, which allows us to determine the replicative life curves (RLS) of yeast cells, curves that provide information on cellular aging. . In conclusion, the chip design, assembly worked, materials used, curves obtained, analysis breakdown and mathematical treatment are presented.