Expresión del cotransportador sodio/yodo (nis) en células derivadas de cáncer de colon: análisis de su efecto en el metabolismo glucolítico celular

Abstract

NIS protein is a glycoprotein located in the basement membrane of thyrocytes, which catalyzes an active iodide transport into the cell. In humans this protein is also thyroid, mammary gland in pregnancy and lactation periods in salivary glands in the gastric mucosa, stomach, breast and ovarian expressed. Previous studies have shown that transfection of NIS in cells derived from different cancers allows the use of radioisotopes of iodine as an effective tool for diagnosis and treatment of such diseases, similar to as is currently used for diagnosis and treatment of thyroid diseases. However, for optimal use of this new type of treatment it is important to understand how the introduction of this protein may affect the metabolism of cancer cells. In the SHOT-Matos (Faculty of Medicine, University of Nice, France) unit, a cell line of colon carcinoma (HT29) stably expressing the nis gene was developed (HT29-NIS). From this cell line, in this study the effect of transfection of the NIS protein on the glycolytic metabolism of HT29 cells under normoxic and hypoxic conditions was assessed by comparing the viability and proliferation, and glucose uptake between HT29 and HT29-cells using NIS probe 2-NBDG glucose. Also in this study the expression of some genes involved was analyzed in cell glycolytic pathway as GLUT-1, HK-1 and PK-M2 by RT-PCR. The results determined that the viability of the two cell lines is not significantly affected by the culture time. Regarding glucose uptake was found that the metabolism of cells is heterogeneous, but at 24 hours after exposing cells hypoxia this metabolism tends to be homogenized as the larger proportion of the cells tend to capture the same glucose concentration, on the other hand it was found that the cell line HT29-NIS captures a greater glucose concentration that the cell line HT29-WT both normoxia and hypoxia. As for the transcription of the genes analyzed increased expression of each in both cell lines after treatment with hypoxia it was observed, with cells transfected with NIS exhibiting a greater increase transcription of genes relative to the silvestres.Expresión cells sodium / iodide symporter (NIS) in cells derived from colon cancer: analysis of their effect on cell glycolytic metabolism