Evaluación de la Expresión del Gen NCED, que Codifica para la Enzima 9-Cis Epoxicarotenoide Dioxigenasa, en Tubérculos de Mutantes Sólidos (Irradiados con Cobalto 60) de Solanum Tuberosum Grupo Phureja, Variedad Criolla Colombia

Abstract

Studies carried out in the Molecular Biology research group (BIOMOLC) of the curricular project of the Biology Degree, of the Francisco José de Caldas District University (Bogotá, Colombia), within the framework of the technical cooperation program of the International Atomic Energy Agency ( IAEA, with Solanum tuberosum phureja group tubers, Criolla Colombia variety, have shown phenotypic changes in seedlings that germinated from seeds irradiated with Cobalt 60 (60Co). This research has as a general objective to compare the expression of a gene associated with NCED enzymes in tubers of a culture of S. tuberosum (Phureja Group, Creole variety Colombia) that sometimes went to radiation with 60Co vs tubers of the same variety without irradiating , through an experimental design where to use laboratory protocols such as: polymerase chain reaction (PCR), electrophoresis and reverse transcriptase (RT-PCR), taking samples of various treatments. The NCED genes in Creole potato, is a gene involved in the biosynthesis of abscisic acid; phytohormone involved in the dormancy process, defined as an adaptive process that plants use in a certain period of their growth and development, in which they suspended control of their physiological activity due to both biotic and abiotic factors allowed, the energy reserve for Survival against agents that prevent or delay your vegetative cycle