Evaluación del larvicida de extracto de Cannabis Sativa sobre larvas de cuarto estadio de mosquito Aedes Aegypti en condiciones de laboratorio

Abstract

Currently, one of the most serious problems in public health is the increase in the distribution of Aedes aegypti in America, since to date, it is the only transmitter of the dengue virus, this and other diseases are produced by the bite of this mosquito, due to its easy adaptation to the environment for its reproduction. Therefore, a larvicide based on Cannabis Sativa was developed to control the proliferation of this vector. The main objective is to determine the optimum concentration of Cannabis sativa extract for the mortality of Aedes aegypti larvae. For this purpose, they evaluated and tested 7 concentrations of Cannabis Sativa extract obtained by means of the Soxhlet extractor and then the rotoevaporator. The results showed that for the biological control of A. aegypti IV stage larvae in laboratory conditions, the optimum concentration is 600 ppm, since it achieved 100% lethality of thelarvae after 12 hours of exposure to the extract, while at a concentration of 1ppm, 63% lethality of the individuals exposed to the extract was achieved after 48 hours. The analysis by means of linear regression showed that the exact concentration to reachthe LC50 of the sample in A. aegypti is 0.2 ppm of the extract and to achieve the LC90 the individuals must be subjected to a concentration of 544 ppm, to achieve the effectiveness of the extract of C. sativa as a larvicide.