Análisis de la expresión del gen Nbp35 en la ruta del jasmonato del cultivo de Solanum Tuberosum Vf. Phureja irradiada con Co60

Abstract

The present work realised under the modality of investigation and innovation in the line of: Genetics of the conservation, with the group of Investigation: molecular biology (BIOMOL) of the University Distrital “Francisco José de Caldas”, deepen in the study of the species Solanum Tuberosum vf. Phureja, with the end to analyse the expression of the gene NBP35 pertaining to the route of the jasmonato radiated with Co60 situated in the municipality The Rosal, farm “el pino” km 16 road Subachoque Cundinamarca, contributing of this form to the study of the present genes in the species to treat, also observed the presence or absence of the gene related with the dormancy, present in the route of the jasmonatos, by extraction of the DNA and RNA of Solanum tuberosum vf. Phureja, in means taken part in the laboratory and of this same form examined with PCR the expression of the present gene in the mutant candidate of Solanum tuberosum vf. Phureja In a fragment of RNA splitting of an only copy of this original fragment, It considered that the investigation provide the necessary data that show an expression of the gene NBP35 involved in the dormancy, with the end to give to know in more detail the level of expression of this in the process of dormancy regarding the natural species, likewise corroborated with the empirical data in base to the dormancy that possesses the plant, noticing of this way a change to level phenotypic in relation with the level of expression of the gene NBP35 in plant control and the plant radiated with Co60, to finalise obtained a correct correlation with the results of the QPCR, taking of data phenotypic and application of the statistical test of ANOVA.