Contribución al Estudio de la Oruga Lonomia descimoni

Abstract

The advent of proteomics, based on the separation of proteins, and analysis techniques more and more sensitive and fast in mass spectrometry (MS), opens up new opportunities for the complete characterization of complex biological samples. The application of proteomic tools to animal venoms has resulted in a dramatic expansion of knowledge of its composition in a growing number of species around the world. Venomics studies are abundant in snakes, but in other animals like scorpions, spiders, centipedes and snails, are scarce. The research of them venoms of species belonging to the Lepidoptera order is limited to the analysis of the bioactivity of the crude or of fractions of these venoms, and not there are reports of research focused in the composition (proteins constituents). This work is a contribution to the study of the composition of the species Lonomia descimoni. The venoms crude is analyzes through a separation of the proteins by electrophoresis SDS-PAGE, tryptic digestion , high efficiency liquid chromatography in reverse phase (RP-HPLC) coupled to mass spectrometry in tandem and finally sequencing and comparison in databases of proteins.