Efecto de Metabolitos Secundarios de Ageratina vacciniifolia Sobre el Potencial Regulador de la Frecuencia Cardíaca Y Regenerador de la Aleta Caudal del Modelo Animal Pez Cebra

Abstract

Cardiovascular disease is the leading cause of death in Colombia and the world. In order to counteract this situation, the search for pharmacological alternatives has been developed, trying to find solutions in vegetal sources. Ageratina vacciniifolia is a plant which is thought to have a regulative potential of blood pressure and in cardiac tissue repairing. In relation of what has been mentioned above, the aim of this study is to evaluate if the total ethanolic extract from Ageratina vacciniifolia has a potential regenerative effect on the caudal fin and as an stabilizer of the heart rate in zebrafish (Danio rerio).The zebrafish model does not have studies in Colombia and is of great importance in the study of biological activity of natural sources. This work allowed to establish values of LC50 under the protocol of the (OECD). The value obtained for Artemia salina was 39.07mg/L, while in the zebrafish larvae was 3.37 mg/L. With respect to the morphological effects, the concentration of 3mg/L caused: microphthalmia, pericardial edema, diminished heart rate, body curvature, diminished body length, muscular degeneration, absence of touch sensitivity and necrosis. In the regenerative assay, we observed a bimodal behavior depending on the concentration. At 2mg/L the regenerative potential increases, while at 2.5mg/l the regenerative potential was inhibited and occurred a healing process compared with the control. In relation to the heart rate, all the concentrations except 0.5 mg/l present a decrease compared to the control.