Diseño e Implementación de un Prototipo Biomédico para la Determinación de la Incidencia de la Radiación UV, Sobre las Características Eléctricas de Tejidos Celulares

Abstract

Endorsed methodologies for determining the sun-protection product’s effectiveness, use healthy volunteers who are exposed to radiation. To find an alternative to this type of evaluation, it was investigated in the pig skin model ex vivo the effect of exposure to UV (Ultraviolet) radiation on the electrical properties of tissue, evaluated in terms of energy and impedance, for establishing a mathematical relation’s model of electrical change versus time of exposure; to achieve this goal, an equipment was implemented using the four electrodes measuring configuration, wherein two needle electrodes supplied with a bipolar signal electrostimulation in burst mode, the other two silver cup electrodes, are implemented to acquiring signals from the tissue. Results in a decrease of energy response and impedance values after exposure is obtained, this behavior is directly associated in the literature to damage tissue structure. The results show that it is possible to establish the effect caused by radiation in terms of the electrical properties of an ex vivo tissue, making it a promising strategy to establish the level of SPF (Solar protection factor) of a cosmetic formulation.