Expresión De Ppar-Γ, Nf-Κb, Iκb, Y Cox-2 En Vellosidades Aisladas De Ratón Adulto Icr Infectadas Con Rotavirus Ecwt Y Tratados Con Agonistas De Ppar-Γ Y Ácido Retinoico

Abstract

Rotavirus is the principal cause of diarrhea children under 5-year-old minor of age and in developing countries it causes 453 000 million deaths per year. Although actualy exist two vaccines on the market of rotavirus alive attenuated (Rotarix ™) and (RotaTeq ™) that reduce the symptoms associated with the infection, has been observed that these do not disappear completely and even they present hospitalization due to the infection for rotavirus. For such a motive in the Laboratory of Molecular Biology of Virus of the National University of Colombia there are looked therapeutic alternatives that allow reduction of the infection, trough the study of cellular molecules like ROS, NF-kB, PPAR-γ And COX-2 which would induce an inflammatory response in the host cell as mechanism of defense before the attack to the virus. It has be found that stimulation of PPAR-γ with the medicament agonista Pioglitazona and the treatment with antirust as N-acetilcisteína (NAC) reduces the infection, nevertheless it has not decided if this effect is due to the direct action of the medicaments on the virus or to PPAR's activation and consequently of pro-inflammatory routes. The objective of this Word has been determine the effect of PPAR's agonist Pioglitazona, Tiazolidinediona, Rosiglitazona, linoleic acid on the expression of PPAR-γ, IκB, NFκB and COX-2 use as cellular model villi of adult mouse ICR infected with rotavirus ECwt, which were isolated, infected and then treated or not with PPAR's agonistas and the retinoic acid. The infection and the expression of the cellular proteins was evaluated using inmunocitochemistry, inmunofluorescence, Western Blot and ELISA of capture. In all the cases, the levels of expression of protein were compared with his corresponding controls not agreements. It was determined thah rotavirus infection on insolated villi of ICR adult mouse increases the expression of PPAR-γ, IκB, NFκB, COX-2 and Hsc70. The PPAR's agonist and retinoic acid decreases rotavirus infection in insolated villi of ICR adult mouse and modify the expression of the proteins PPAR-γ, NF-κB, IκB, COX-2 y Hsc70 (returning to baseline levels).