Caracterización de isotermas de presión superficial en función del área, para películas interfaciales del sistema tensioactivo pulmonar

Abstract

The structural studies and biochemical characterization of pulmonary surfactant, are developed from material obtained from bronchoalveolar lavage of animal origin (Serna, 2006), since it is in the lung where the gas exchange is carried out providing the necessary oxygen for respiration and Hence from where the pulmonary surfactant is withdrawn. To carry out this biophysical study of the interfacial films of pulmonary surfactant, compression isotherms of porcine origin were made from bronchoalveolar lavages; The amount of phospholipids contained in the material (Bligh, 1959) is evaluated by measuring the surface pressure as a function of the air-liquid interface area at two reference temperatures 25 ° C and 37 ° C . These measurements take into account the variation of factors such as: the mass of the surfactant (10μg, 25μg and 50μg) applied at the interface, and the film compression rate (37mm/min, 70mm/min and 100mm/min), using a surface tension balance or KSV Nima surface balance, which basically consists of a Teflon vessel in which an aqueous subphase of monolayer buffer is placed on which the surfactant forming the interfacial film is deposited at analyze.