Termometría de fluorescencia para hipertermia con nanopartículas: Relación entre la intensidad de fluorescencia y la temperatura de un fluoróforo

Abstract

Therapeutic hyperthermia is a promising technique for cancer treatment that uses controlled temperature rise to kill tumor cells, but its application presents challenges in terms of accuracy and temperature control. Fluorescence-based thermometry has gained attention for its ability to make precise and localized measurements, especially in combination with plasmonic nanoparticles. The objectives of this work were to understand the physics related to temperature-dependent fluorescent phenomena, to determine the most appropriate fluorescence thermometry methods for measuring the temperature of nanoparticles inside cells, and to implement a mathematical model to quantify the temperature of nanoparticles based on the fluorescence intensity of a fluorophore. The methodology included a review of the physics of fluorescent phenomena, various methods of fluorescence-based thermometry, and the development of a mathematical model based on Lindblad's master equation. The results established a quantitative relationship between temperature and decay rates in two-level fluorescent systems, demonstrating that increasing temperature increases the Planck distribution function, which translates into an increase in the total transition rate, directly affecting the quantum yield and excited-state lifetime of fluorescent molecules.