Prospección molecular de ADN antiguo en muestras arqueológicas óseas y dentales de origen humano

Abstract

The recovery of biomolecules from ancient biological samples is almost impossible; however, different investigations have aimed to make efforts to define methodologies at morphological, histological and molecular scales for the optimal analysis of this type of samples, especially in relation to the extraction of DNAa, although it is highly fragmented and in low concentrations (Haynes & Searle, 2002; Pilli et al., 2013; González Espino, 2023; Corach & Sala, 2001; Rodríguez Saza, 2019). Therefore, the present project aims to carry out molecular prospecting for DNAa in bone and dental samples of human origin obtained from archaeological contexts, analyzing and detailing their level of biochemical preservation through various tests and techniques. Finally, the mitochondrial haplogroup belonging to the studied individual, through locus 16132-16217 of the hypervariable region 1 (HVR1).