Variación genética de callos de Solanum Phureja en in vitro con Kinetina y ANA

Abstract

In this research work, the androgenic behavior of candidate MV6 mutant clones of Solanum tuberosum L., belonging to the Phureja Group, was evaluated. The effect of the balance of the phytohormones auxin (ANA) and cytokinins (Kinetin) in the Murashige and Skoog (MS) culture medium was analyzed to assess the androgenic capacity of the mutant clones. Calluses were generated from pollen (microspore) and the influence of phytohormones on the callogenic process was examined, stimulating both cell proliferation and differentiation in the callus cells present in the mutants of Solanum tuberosum L., Phureja Group. However, callus formation was not achieved as a result of the regeneration process. Although the pollen showed high viability, an embryogenic inability was observed in the callus due to the accumulation of oxidized polyphenols in the cells. It is important to note that the use of mutant clones can cause greater fragility in the anthers of Solanum Phureja, which affects the genes responsible for the production of tissues and cells in the anthers, decreasing cellular structural strength and, therefore , preventing the regeneration of calluses.