Efectos Morfológicos Inducidos en Berenjena (Solanum Melongena L.) Variedades Co015 Y Ph10 por la Aplicación de un rango de dosis de Radiación Gamma

Abstract

In order to find a range of doses to induce interest mutations , the radiosensitivity curve for Solanum melongena L. varieties CO015 and PH10 was made by the report and classification of morphological effects caused by exposure of the seeds to acute doses gamma radiation in a range between 0 and 600 Gy, using Co60 as a source. The varieties used are product of improvement programs developed for this species at the University of Córdoba, by plant breeders Hermes Aramendiz and Miguel Espitia. The seeds were distributed according to their variety in a control group and six different treatments of 100 Gy, 200 Gy, 300 Gy, 400 Gy, 500 Gy and 600 Gy. 3000 seeds approximately were irradiated at the irradiation unit Cobalt 60 source type C-188 of the CNEA (INGEOMINAS). Posttreatment growth was induced under controlled conditions in a completely randomized design of 7x2 with three replications, in order to compare the effect of treatments on seedlings evaluate five variables : the height, the percentage of germination, emergence percentage, the length and width of the leaf during the first 45 days of growth. An analysis of variance was performed to determine whether there were significant differences between treatments and between the overall effects of these on each variety. Then, the height data were adjusted to an independent simple linear regression to determine the appropriate dose which allows reducing the plant habit between 30% and 50% while maintaining physiological stability (Kodym et al, FAO, 2011) . Previously, the appropriate laboratory conditions were evaluated and established of plant biotechnology of the University Francisco José de Caldas, in order to ensure normal growth of seedlings irradiated under homogeneous conditions for 45 days conditions, avoiding the intervention of external factors for data collection of responses, without creating damage or stress to the seedlings during this process. It was found that there are no significant differences between the response of the height between the varieties studied, then reporting the LD50 above 400 Gy. In addition, based on data regression it was determined that a dose range between 280 and 370 Gy reduced the plant habit between 30% and 50% taking as reference the variable seedling height at 19 days .