Evaluación de la susceptibilidad de larvas del IV estadio de la especie Aedes Aegypti linaeus (Díptera : Culicidae) a un insecticida alternativo a base del extracto de colillas de cigarrillos en condiciones de laboratorio

Abstract

In the present study the effect was evaluated larvicidal and the susceptibility of the larval of the stadium IV of the mosquito Aedes aegypti to the extract obtained of the stubs of cigarette in concentrations of 250, 500, 750 and 1000 ppm in laboratory conditions. The tests fulfilled in the laboratory of zoonosis of the University Distrital Francisco Jose of Caldas, under the methodology of Bobadilla's protocol. 4 concentrations took 250, 500, 750 and 1000 ppm as reference, for every concentration 5 containers took: a container like witness and in other four repetitions for concentration. In every container 25 Aedes Aegypti's larvas put in the IVth on stadium and monitorings were realized at 1 a.m., 12, 24, 36 and 48 hours. There was obtained that the concentration of 1000 ppm registered a major effect larvicidal with a maximum mortality 83 % which was reached in the first 48 hours, the following concentration of 750 ppm obtained a mortality of 64 % in 48 hours; as for the lowest concentrations 500 ppm there is reached a mortality of 37 % and in the concentration of the 250 ppm a mortality of 7 % after 48 hours of follow-up. Spent 15 days there was realized the first evaluation of residual effect in the concentrations of 250, 500, 750 and 1000 ppm repopulating all the containers and monitoring in the same frequency, obtaining like proved a mortality of 51 % in the concentration of 1000 ppm, in 750 ppm a mortality of 24 %, in the concentrations of 500 there was reached a mortality of 19 %, and 250 ppm here was reached a mortality of 4% in a period of 48 hours. To 30 days there was realized the second evaluation of residual effect following the same procedure and the same frequency reaching this way in the 1000 ppm a mortality of 16 %, in the 750 ppm a mortality of 11 %, as for the lowest concentrations 500 ppm there is reached a mortality of 6 % and in the 250 ppm a mortality of 4 % in the first 48 hours of treatment.