Producción, Aislamiento y Evaluación de la Estabilidad de Vesícula de Membrana Externa Provenientes de una Cepa de E.Coli JC8031-pTRC-ssTorA::gfp

Abstract

Negative Gram bacteria produce outer membrane vesicles (OMVs) and these can contain nucleic acids, biologically active proteins, enzymes, toxins, antigens among other molecules. In addition to their role as carriers, OMVs also play a very important role in bacterial survival: their production is regulated by a period of stress due to significant nutritional acquisition, interaction with the environment, biofilm formation and pathogenesis. Unlike other secretion mechanisms, OMVs activate the bacteria to secrete insoluble molecules in addition to a soluble material complex. On the other hand, the green fluorescent protein (gfp), is considered as a biomarker, because thanks to the fluorescence allows to have data about the location behaving like reporter genes because they provide information of the biochemical changes Inside and between the cells. For this particular case, the previously named marker was used in order to evaluate the stability of the OMVs thanks to the intensity of electromagnetic radiation that it emits.