Evaluación del efecto larvicida del extracto acetónico de rosmarinus officinalis (romero) sobre Aedes aegipty, mediante dos métodos de extracción

Abstract

This study evaluated the larvicidal activity of the Rosmarinus Offinalis (Rosemary), applied on Aedes aegypti IV stage larves, using two differents extraction methods. In order to develope this investigation, five acetone extracts were used by each extraction method (extraction with soxhlet equipment and extraction by maceration), at concentrations of 500 ppm, 1000 ppm, 1500 ppm, 2000 ppm and 2500 ppm. Four trials were done by biological assay, each with 25 larves, exposed by 48 hours and making larval mortality counting every twelve hours (12, 24, 36 and 48 hours). Biological assays were developed using OMS protocols and control groups. The most toxic effect was evident in hot extract (soxhlet equipment), with a mortality of 100% at 12 hours and 2500 ppm concentrations, showing better results respect to cold extract (maceration), which had a 100% mortality but after 48 exposure hours. Lethal concentrations 50% (CL50) and 95%(CL95), for hot extraction were 1161,94 ppm and 1889,08ppm, and for cold extract were1296,62 ppm y 2507,72 ppm, respectively. In order to verify statistical differences, comparisons of each extraction method were done separately by ANOVA analysis and to compare both extractions methods, they were submitted to a MANOVA analysis.