Cuantificación de cefalexina y amoxicilina en suelos en la ribera del río Bogotá

Abstract

Amoxicillin and cephalexin are compounds that can persist in soil for long periods. Its accumulation in the deeper layers of soil can potentially increase the risk of groundwater contamination, affect the organisms that depend on these ecosystems and have long-term implications. For this reason, this study focused on the detection and quantification of these beta-lactam antibiotics in soils on the banks of the Bogotá River at different distances and depths using high-performance liquid chromatography (HPLC). This procedure consisted of using a C18 column with a UV detector at 247 and 260 nm and a mobile phase composed of a mixture of water and acetonitrile (95:5 v/v) in isocratic mode to treat the soil samples with a phosphate buffer solution pH 5.8, which were filtered for preparation. Additionally, exclusive standards were made for each active ingredient and the respective dilutions of the samples for correct reading on the HPLC. The results obtained were effective and reliable for the analysis of soil samples, since the presence of high concentrations of amoxicillin was found, on average 0.554 mg/mL, which indicates concentrations higher than those allowed by the EPA; However, the presence of cephalexin was not detected in the samples analyzed.