Evaluación de la respuesta de expresión de los genes RH2A, RH2B, RH1, RH4, RH5, EBA140, EBA175 Y EBA181 del parásito P. Falciparum en presencia y ausencia de péptidos inhibitorios de interacción Eritrocitaria

Abstract

The internship work is framed in a macro project that aims to study the host immune response against the Plasmodium falciparum parasite, which causes the malaria disease, one of the main threats to public health in the world; as well as the search for possible vaccine candidates based on inhibitory synthetic peptides. For this, it is necessary to study the effect that these molecules have on the parasite, in particular, the mechanism by which they can produce their effect. For this reason, an analysis of the basal expression of the parasite's rh2a, rh2b, rh1, rh4, rh5, ba140, eba175 and eba181 genes, which are involved in the expression of ligand proteins that interact with receptors located on the cell surface of erythrocytes, will be carried out, in the absence of any type of stress, and will be compared with the expression of these genes after treatment with erythrocyte interaction inhibition peptides (1771, 1815, 1818, 34203, 34243). The work will include the generation of a continuous culture of the parasite, synchronization of the parasite cells, RNA extraction, RNA retrotranscription and DNA amplification by real-time PCR techniques. The results obtained will be compared with standard patterns and linear regression will be used to analyze sample expression levels.