Evaluación de la actividad biológica de Cladosporium cladosporioides como alternativa de biotransformación de aceite de recambio de automóvil.

Abstract

The different human activities carried out on a daily basis generate waste that can be harmful not only for human health but also for the environment, such is the case of RESPEL hazardous waste, among which are automotive replacement lubricating oils (CORTOLIMA, 2016). There are different bioremediation processes that represent an unconventional option, and allow transforming compounds or reducing their polluting power by using microorganisms (Guerrini-Schenberg, 2010). In this work, tests were carried out with a strain of black fungus Cladosporium cladosporioides in three liquid media; RC1 which contained C. cladosporioides inoculum, 1% replacement oil, peptone, minimal salts, RC2 with culture medium, inoculum and no replacement oil (blank or control) and RC3 with culture medium, 1% replacement oil and no inoculum (blank or control). These were placed in orbital shaking at 180 rpm and also exposed to a temperature of 25°C using a portable heater. Ten samples were taken every 3 days for 30 days to determine the biotransformation efficiency of the replacement oil using the methanol-chloroform-water extraction method. The results obtained prove the biotransformation of the oil in RC1, which demonstrated the high capacity of the black fungus to reduce this contaminant.