Estudio de asociación de las variantes de los genes MOBP (rs864643), GPC6 (rs7995215), BTBD3 (rs6131295), DRD4 (VNTR Exon III 48pb), HTTLPR (VNTR Exon XV 44pb) Y SLC1A1 (rs301434) en pacientes infantiles colombianos que presentan diagnóstico de trastorno de comportamiento

Abstract

Behavioral disorders are characterized by the persistence of symptoms such as aggression and difficulties on family, social relationship, and academics. The diseases related to these disorders in Colombian children and teenagers are the main reason for consultation in mental health at schools. Recent studies have established associations of some genetic markers with the behavioral disorder such as attention deficit hyperactivity disorder (ADHD), obsessive-compulsive (OCD), autistic spectrum (ASD). In concordance with the above, we were how aim to perform a molecular and statistical genetic analysis from the association study of six genetic markers (MOBP (rs864643), GPC6 (rs7995215), BTBD3 (rs6131295), DRD4 (VNTR EXON III 48pb), HTTLPR (VNTR Exon XV 44pb) and SLC1A1 (rs301434)). Therefore, we analyzed a sample of 50 Colombian children from 6 to 16 years old, with a diagnosis of behavioral disorders, which were compared with 50 controls. The genetic analysis was carried out by conventional PCR (polymerase chain reaction) and real-time RT-PCR (TaqMan probe). The statistical analysis by means of the PLINK program allowed us to make a determination of the Hardy-Weinberg equilibrium, establish the p-value and Odd Ratio of association of the study variants. The results obtained permitted us to conclude that there is no association of any of the analyzed markers with childhood behavior disorders, since they presented p-values, p> 0.05. On the other hand, all markers were found in Hardy Weinberg equilibrium, except SLC1A1. However, all markers presented an Odd Ratio greater than 1, which enabled us to infer that there is a probability of a significant association with the behavioral disorder such as ADHD, OCD, and ASD. This study will allow the analysis and study of genetic markers, also we recommended to expand the study population sample to obtain results that are more significant.