Efecto de la presión mecánica en el atrapamiento celular en el ciclo de envejecimiento de poblaciones Single-Cell de S. cerevisiae

Abstract

Aging is a complex and important biological phenomenon to understand the structure of life, defined as a natural process in which many cellular functions change, evidencing deterioration due to the passage of time. This has been a field of great interest for study since slowing down aging means lengthening the life cycle and preventing many diseases that are generated as a result of it. In this work, specific results were obtained on the incidence of mechanical pressure on yeast cells in aging studies at the “Single-Cell level” by means of a microfluidic device called Mother Machine, which allows the analysis of aging and its changes over time by varying the pressure along with other parameters. The aging study is performed at both chronological and replicative levels. The execution is carried out in stages ranging from the construction of the microfluidic device, obtaining the cell sample to be studied, the experiment and obtaining the data to be analyzed, and finally the processing and analysis of the results obtained at the replicative level. In the second line of analysis, the diffusion in the cell as a result of chronological aging is analyzed with specific experimental arrangements with GEM'S.