Examinando por Materia "Larvas - Crecimiento"
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Ítem Evaluación de la eficacia de los extractos naturales de Eucaliptus Globulus y Platycladus Orientalis como repelente natural contra mosquitos adultos de la especie Aedes AegyptiZubieta Giraldo, Angelica Lizet; Morales Olaya, Leidy JohanaThis research project focuses on the evaluation of the repellent effect of plants: Eucalyptus globulus (Eucalyptus) and Platycladus orientalis (Pino Book) on Aedes aegypti, vector of great importance in public health. The extraction method used by hot soxhlet equipment in use of the essential oils of the plants specified above. For determining the repellent effect bioassays were conducted with 10 volunteers, in order to identify the most effective repellent action, the protection time and comparison between ethanol extracts of Eucalyptus globulus and platycladus in concentrations of 0.2%, 0.4% and 0.6%, each volunteer was exposed to 50 female A. aegypti, the procedure was performed according to the guidelines for testing the efficacy and mosquito repellents on human skin of the World Health Organization (WHO) (WHO, 2009 ). The study demonstrated a repellent action against Aedes aegypti, at concentrations of 0.4% and 0.6% of Eucalyptus globulus and Platycladus orientalis with protection times of 2 hours for each extract.Ítem Evaluación de la susceptibilidad de larvas del IV estadio de la especie Aedes Aegypti linaeus (Díptera : Culicidae) a un insecticida alternativo a base del extracto de colillas de cigarrillos en condiciones de laboratorioTorres Contreras, Mónica Judith; Medina Cardona, Aaron Alexis; Corradine Mora, Diego TomásIn the present study the effect was evaluated larvicidal and the susceptibility of the larval of the stadium IV of the mosquito Aedes aegypti to the extract obtained of the stubs of cigarette in concentrations of 250, 500, 750 and 1000 ppm in laboratory conditions. The tests fulfilled in the laboratory of zoonosis of the University Distrital Francisco Jose of Caldas, under the methodology of Bobadilla's protocol. 4 concentrations took 250, 500, 750 and 1000 ppm as reference, for every concentration 5 containers took: a container like witness and in other four repetitions for concentration. In every container 25 Aedes Aegypti's larvas put in the IVth on stadium and monitorings were realized at 1 a.m., 12, 24, 36 and 48 hours. There was obtained that the concentration of 1000 ppm registered a major effect larvicidal with a maximum mortality 83 % which was reached in the first 48 hours, the following concentration of 750 ppm obtained a mortality of 64 % in 48 hours; as for the lowest concentrations 500 ppm there is reached a mortality of 37 % and in the concentration of the 250 ppm a mortality of 7 % after 48 hours of follow-up. Spent 15 days there was realized the first evaluation of residual effect in the concentrations of 250, 500, 750 and 1000 ppm repopulating all the containers and monitoring in the same frequency, obtaining like proved a mortality of 51 % in the concentration of 1000 ppm, in 750 ppm a mortality of 24 %, in the concentrations of 500 there was reached a mortality of 19 %, and 250 ppm here was reached a mortality of 4% in a period of 48 hours. To 30 days there was realized the second evaluation of residual effect following the same procedure and the same frequency reaching this way in the 1000 ppm a mortality of 16 %, in the 750 ppm a mortality of 11 %, as for the lowest concentrations 500 ppm there is reached a mortality of 6 % and in the 250 ppm a mortality of 4 % in the first 48 hours of treatment.Ítem Evaluación del efecto larvicida del extracto acetónico de Eucaliptus Globulus en diferentes concentraciones en larvas de IV estadio de Aedes Aegypti en condiciones de laboratorio C.Arias Garzón, Yeny Feryohana; Corradine Mora, Diego TomasHe larvicidal effect of acetone extract of Eucalyptus globulus on stage IV larvae of Aedes aegypti in laboratory conditions to test its effectiveness was evaluated. 5 treatments with 4 replicates, each containing 25 specimens with their respective controls were applied concentrations of 100, 250, 500, 750 and 1000 ppm were performed. The results showed that the most effective treatment was 1000 ppm at 87% cumulative mortality of larvae at 48 hours, in contrast to the 100 ppm treatment which exhibits toxicity only 9% in the same time. It was observed that the time interval having the most significant mortality was between 24 and 36 hours in the 5 treatments applied also it found that increasing the concentration of the treatment increased mortality. To evaluate the residual effect of the treatment, it was observed that at 10 days post-acute treatment, but the concentration of 1000 ppm produced a mortality of 43%, while the 20-day mortality was reduced by nearly half, is past say 30 days is no longer any residual effect on the means of implementation of the extract, indicating biodegradation and disappearance of the toxic effect.Ítem Evaluación larvicida del extracto etanólico de la semilla de carica papaya sobre larvas del IV estadio de Aedes Aegypti (Diptera : Culicidae) en condiciones de laboratorioGómez Gutiérrez, Natalia Rocio; Corradine Mora, Diego TomásThe following project aims to evaluate the larvicidal effect of ethanol extract of Carica papaya seeds on Aedes aegypti larvae of IV stage in laboratory conditions, to generate natural alternatives on controlling the dengue vector. In the methodology were conducted six bioassays with different concentrations of ethanol extract of Carica papaya seeds 50, 100, 250, 500, 750 and 1000 PPM with four repetitions each, using 100 specimens by bioassay and a control group that only got dechlorinated water to compare mortality in the larvae subjected to experimental treatments and natural mortality without exposure. Readings were taken at 0, 2, 12, 24, 36, and 48 hours post exposure for the observation of mortality in each of the bioassays. Subsequently it conducted an evaluation of residual effect of C. papaya extract with fortnightly repopulation of specimens in the same initial conditions and thus determine the toxicity decrease of the extract diluted in water with the passage of time. Mortality rates in the bioassays of the ethanolic extracts of Carica papaya seeds in 50, 100, 250, 500, 750 y 1000 PPM after 48 hours were 46%, 87%, 95%, 99%, 100% y 100% respectively. It was determined by the probit model LC50 48.8 ppm and 151.2 LC 90 PPM. For lethal times 50% (TL 50) and 90% (TL90), it was found that TL50 was between 5 to 8 hours and TL90 between 10 to 12 hours , wherein the lethal time decreases by increasing the concentration. Finally it was corroborated through ANOVA analysis of a factor that the concentrations affect the mortality of Aedes aegypti larvae and it is concluded that the extract of Carica papaya has a high larvicidal effectiveness in concentrations above 100 ppm. As for the residual effect the mortalities were lower than initial, with 0%, 8%, 11%, 14%, 19% y 23% respectively to the aforementioned concentrations, therefore the extract degradation is evident with the passage of time, ensuring the use of this in aquatic environments for control of Aedes aegypti.