Determinación de bacterias en el aire del laboratorio de microbiología de la Facultad de Medio Ambiente y Recursos Naturales de la Universidad Distrital Francisco José de Caldas asociadas a posibles afecciones en la salud

Abstract

This research has been conducted to find possible exposure to airborne bacterial pathogens, and evaluate potential risks to human health who performing activities at the Microbiology Laboratory of Environment and Natural Resources Faculty at the Distrital Francisco José de Caldas University. Air samples were taken by the sedimentation technique in Nutrient Agar (NA) for aerobic bacteria and Sulfite Polymyxin Sulfadiazine (SPS) for anaerobic bacteria in two points of the laboratory, different times with different activities. After incubation to 37C for 48 h., the results were reported as UFC/15min/63.61cm2, and the pure cultures of recuperated colonies were characterized with Gram strain and colony morphology. The isolated bacteria were re-cultured in selective and differential agars and finally they were identified using the BD BBL Crystal system. The results showed that the number of Gram positive bacteria were greater that Gram negative bacteria, Micrococcus sedentarius, Staphylococcus sp., S. haemolyticus, S. capitis, S. cohnii sp. Cohnii, S. hominis and S. lentus. Likewise, Leuconostoc pseudomesenteroides, Bacillus subtilis and Corynebacterium renale Group. associated with pulmonary infections and allergic reactions. Gram negative bacteria were identified as Pseudomonas sp., P. stutzeri and P. Putida, Yersinia pseudotuberculosis, Y. enterocolitica Group., Serratia fonticola, Shigella sp., Klebsiella pneumoniae sp. ozaenae, Citrobacter freundii and Acinetobacter iwoffii, cause respiratory infections and lymphadenitis.