Normalización de la amplificación mediante qPCR de los genes ID1 e ID3 a partir de biopsias de adenocarcinoma gástrico embebidas en parafina y línea celular AGS
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In Colombia, by 2018, gastric cancer (GC) had a high incidence and mortality rate, being the fourth most common cancer in both sexes. Overexpression of ID1 and ID3 in various types of cancer, including gastric cancer, accounts for their role in tumorogenesis in various tissues. Studies show that CG overexpression of these genes is related to the carcinogenic potential of gastric tumor cells, poor differentiation, advanced stages of disease and increased tumor aggressiveness so that these genes could be considered as molecular markers for treatment, forecasting, and disease prevention. Pathology departments archive a large number of paraffin embedded specimens fixed with formalin (FFPE), material that represents an available resource and little used in the study of biomarkers, because the chemical properties of RNA and DNA change in the formalin binding process. The objective of this work was to normalize a methodology for the amplification of the ID1 and ID3 genes from RNA obtained from gastric cancer FFPE biopsies and the AGS cell line. This study included paraffin-embedded biopsies of some GC patients treated at the National Institute of Cancerology (INC), RNA was extracted from FFPE sections, then submitted to Preamp and amplified by qPCR, finding that without the use of Preamp, the stability of the constituent gene GAPDH was better than when it was used, but the threshold cycle occurs later without that treatment for the ID1 and ID3 genes. Additionally, cytotoxicity tests with Cannabidiol are tentatively performed on the AGS cell line because this compound has antitumor and negative-regulation properties for the ID1 gene.