Please use this identifier to cite or link to this item: http://hdl.handle.net/11349/14443
Title: Caracterización química de telaraña de la especie leucauge acuminata
Author: Avellaneda Gálvez, Sara Juliana
Advisor: García García, Alexander
Carazzone, Chiara
Subjects: Licenciatura en Química - Tesis y disertaciones académicas
Araña
Telas de araña
Leucauge acuminata
Keywords: Solubilización
Espidroínas
Tela de araña orbitelar
Leucauge acuminata
Electroforésis en Gel
HPLC-MS
Date: 14-Feb-2017
Abstract: The properties of spider webs are highly valued and can have several applications. In order to understand and take advantage of these properties it becomes necessary to study and characterize its composition, Who, According to the previous studies, it has been described that they consist mainly of large fibrous proteins and some biomolecules that cover the threads and have complementary functions to those of the silk, But this is only possible by having the fibers in solution, so this prior process is essential. In addition the recognition of the components of spider webs of different species contributes to expand the perspective of the use of these biomaterials, their functions in the ecosystem and in the life of the spiders that make them. This project aims to characterize these components from spider webs already made by the species Leucauge acuminata, to solubilize them in different solvents and to use analytical methods such as SDS-PAGE and HPLC-ESI-MS electrophoresis in the separation and identification of analytes, especially Proteins.
Description: The properties of spider webs are highly valued and can have several applications. In order to understand and take advantage of these properties it becomes necessary to study and characterize its composition, Who, According to the previous studies, it has been described that they consist mainly of large fibrous proteins and some biomolecules that cover the threads and have complementary functions to those of the silk, But this is only possible by having the fibers in solution, so this prior process is essential. In addition the recognition of the components of spider webs of different species contributes to expand the perspective of the use of these biomaterials, their functions in the ecosystem and in the life of the spiders that make them. This project aims to characterize these components from spider webs already made by the species Leucauge acuminata, to solubilize them in different solvents and to use analytical methods such as SDS-PAGE and HPLC-ESI-MS electrophoresis in the separation and identification of analytes, especially Proteins. Con el fin de comprender y aprovechar dichas propiedades surge la necesidad de estudiar y caracterizar su composición, la cual según estudios anteriores, se ha reportado que consta en su mayoría de proteínas fibrosas de gran tamaño y algunas biomoléculas que recubren los hilos y que tienen funciones complementarias a las de las telas [1], pero esto solo es posible, teniendo las fibras en solución, por lo cual éste proceso previo es de vital importancia. Además el reconocimiento de los componentes de telas de especies diferentes a los ya estudiados contribuye a ampliar la perspectiva con respecto al uso de estos biomateriales, las funciones que cumplen en el ecosistema y en la vida misma de la araña que las produce. En este proyecto se busca la caracterización de dichos componentes a partir de telas de araña ya elaboradas por la especie Leucauge acuminata, mediante la solubilización de la misma en distintos solventes y el uso de métodos analíticos como Electroforesis SDS-PAGE y HPLC-ESI-MS en la separación e identificación de analítos, especialmente proteínas.
URI: http://hdl.handle.net/11349/14443
Appears in Collections:Licenciatura en Química

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